Tronolab protocols

Lentiviral Vectors

The quality of the DNA is important for optimal results. We recommend the nucleobond AX kit from Macherey-Nagel to prepare DNAs although other kits may be satisfactory as well.

• Lentivectors Production pdf
  
• Lentivectors Titration html
  
• RT Assay html
  
• TU vs pg of p24 html
  

siRNA

• Annealing of shRNA pdf
  
• shRNA Cloning Strategies html
  

Molecular Biology

• Kozak Consensus html
  

Genotyping

• Genomic DNA isolation html
  
• Southern blot html
  

Phenotyping

• Fibroblasts isolation html
  

Vector Production EPFL 2007.pdf